TY - BOOK AU - Alexander Badu-Boateng PY - 2018 CY - München, Germany PB - GRIN Verlag SN - 9783668783799 TI - Evaluation of the Ability of Three Commercial DNA Extraction Kits to Remove PCR Inhibitors from Soil UR - https://www.hausarbeiten.de/document/436868 N2 - Crime scene investigation is an important step in the entire criminal investigation process because this is where evidence is gathered. Blood from the perpetrator or victim of a crime can be left at crime scenes or transferred to other materials such as clothing, knives and guns. Most often, this body fluid is contaminated with soil at outdoor crime scenes but this might be the only or the most important evidence in solving a crime. This study aimed at identifying the most appropriate method of extracting quality DNA from soil contaminated blood using three commercial DNA extraction kits (PrepFiler Forensic DNA Extraction kit, Promega DNA IQ Kit, Blood Miniprep kit) that have been claimed by the manufacturers to be effective in extracting quality DNA from soil contaminated samples. Human blood was mixed with soil and stored at room temperature/25℃ for a 12 week period. The PrepFiler kit and DNA IQ kit were successful at removing possible PCR inhibitors from the soil during DNA extraction with no significant difference (p=0.887). The Blood Miniprep kit performed poor in terms of removing possible PCR inhibitors. KW - Forensic Science, Forensic DNA, DNA from Blood, PCR Inhibitors, PrepFiler Forensic kit, DNA IQ kit, Blood Miniprep kit, Real-time PCR LA - English ER -